Figure 6.

Foxo3a regulates miR-633-induced chemoresistance dependenting on FADD in gastric cancer cells.

(a), TUNEL assay in SGC-7901 cells to detect apoptosis after infected with adenoviral Foxo3a. (b and c), inhibition of Foxo3a by transfect with Foxo3a siRNA (si-Foxo3a) or scramble siRNA (Foxo3a-sc) rescued 2 μM DOX induced cell death in SGC-7901. Real-time PCR to detecte miR-633 level, FADD protein expression were detected by western blot (b) and TUNEL assay to detect cell apoptosis in SGC-7901 cells (c). (d and e), overexpression of Foxo3a treated with 0.2 μM DOX for 24 h in SGC-7901 cells, miR-633 level, FADD protein expression (d) and TUNEL assay (e) were detected. (f), the mRNA and protein expression levels of FADD analyzed by qRT-PCR and western blot in SGC-7901 cells after infected with adenoviral Foxo3a. (g), overexpression of Foxo3a elevated FADD protein level in SGC-7901 cells in response to 0.2 μM DOX treatment for 36 h, which reversed by miR-633 mimics. (h), overexpression of Foxo3a increased cell death in SGC-7901 in response to 0.2 μM DOX treatment for 36 h, which was reversed by FADD siRNA. FADD protein level analyzed by western blot. Error bars represent S.D. Data are expressed as the mean ± SD of 3 independent experiments.*P < 0.05.