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. 2017 Sep 29;10(2):146–161. doi: 10.1080/21541248.2017.1356432

Figure 6.

Figure 6.

Liposome-mediated delivery of anti-KBD antibody of Ranbp2 into 661W neurons leads to mitochondrial fragmentation, delocalization of RanBP2 from mitochondria and neuronal atrophy. (A) Ranbp2 colocalizes extensively with mHsp70-immunostained worm-like mitochondria in 661W neurons. Ranbp2 was detected with ZnF Ab09. (B) Ranbp2 colocalizes extensively with mHsp70-immunostained worm-like mitochondria in liposome mock-treated cells and cells present healthy morphology (a-d). Liposome-mediated delivery of KBD Ab41 into 661W neurons (e-h) causes strong neural atrophy (e), mitochondrial fragmentation (g) and the delocalization of Ranbp2 from the mitochondria (h) without affecting the localization of Ranbp2 at the nuclear rim (f). Internalized KBD Ab41 was detected with Alexa-conjugated goat anti-rabbit antibody (f). (C) High-magnification images of liposome mock and KBD Ab41-treated 661W neurons immunostained for Ranbp2 and mHsp70 as shown in image panels of B. Legends: KBD Ab41 Transf., cells with liposome-mediated delivery of Ranbp2 antibody, KBD Ab41; Mock Transf., liposome mock-treated cells. DIC, differential interference contrast (Nomarski optics). Scale bars: 10 μm (A), 30 μm (B), 20 μm (C).