Figure 1.
Depletion of T. brucei RRP44 by inducible RNA interference. (a) Effects on cell growth. T. brucei cells transfected with vector p2T7-177–RRP44 were growth in the presence (circles) or absence (filled squares) of tetracycline. Non-transfected cells in the presence of tetracycline (open squares) were also used as control. Cultures were diluted to 106 cells after 48 hours for the next time points. (b) Analysis of the level of TbRRP44 mRNA by quantitative RT-PCR normalized to GAPDH. Error bars indicate standard deviation of triplicate experiments. (c) Decreasing of RRP44 protein detected on a western blot using polyclonal antiserum at different time-points after RNAi induction.