Figure 3.

Depletion of T. brucei RRP44 impairs cleavage steps related to the LSU rRNA maturation. The relative change in the target amplicons was determined by quantitative RT-PCR analysis of total RNA and normalized to the GAPDH mRNA. Control cells (black bars, normalized to 1), cells 48 h post RNAi induction (dark grey bars) and cells 72 h post RNAi induction (light grey bars). Error bars indicate standard deviation of triplicate experiments. At the bottom, a schematic representation of the rRNA precursor, indicating the amplicons analyzed in this work (black dashes).