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. 2019 Jan 11;16(1):144–153. doi: 10.1080/15476286.2018.1564464

Figure 1.

Figure 1.

PAX3-FOXO1 during human MSC muscle differentiation. (a) shRNA targeting PAX3-FOXO1 (shPF) specifically silenced the fusion RNA (PF), but not the parental genes. The target sequence has been reported before [8]. The levels of the transcripts were measured by quantitative RT-PCR, and normalized to that of an internal control, GAPDH. (b) MSC cells stably infected with the virus expressing shPF resulted in reduced expression of MYOD, and no expression of MYOG and MYH1. (C-E) Overexpressing PAX3-FOXO1 promotes human MSC cells proliferation and survival. Cell proliferation and viability was measured by MTT. Compared with empty vector transfected cells (hMSCs/CT), human MSC cells stably expressing PAX3-FOXO1 (hMSCs/PF) grew faster in growth media (GM) (c), continued to proliferate in differentiation media (DM) (d), and survived more in serum-free media (SF) (e). *, p < 0.05; **, p < 0.01; ***, p < 0.001.