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Pax3-Foxo1 fusion RNA is not detected in sheep, pig, or horse muscle differentiation systems. (a-c) Absence of Pax3–Foxo1 RNA during muscle differentiation in sheep bone marrow MSCs (a), pig bone marrow MSCs (b), and horse ASC cells (c). These cells were induced to differentiate along a skeletal muscle lineage. RNA was extracted from samples harvested every other day from day 2 to day 20. Pax3–Foxo1, MyoD, MyoG, Myh1, and Gapdh RNAs were assessed by RT-PCR.
