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. 2018 Dec 27;16(1):13–24. doi: 10.1080/15476286.2018.1553482

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Figure 5. — Verification of DELs in hNPCs and differentiated neurons and astrocytes. (a) Phase-contrast image of hNPCs derived neurosphere, (b) ICC images of hNPCs showing positive for NESTIN and SOX2 markers, (c) hNPCs derive neurons were positive for neuronal markers MAP2 and TUJ1, (d) hNPCs derive astrocytes were positive for GFAP and S100β, (e) relative expression of significantly upregulated lncRNAs, AQP4-AS1, CYP-AS1 and LHX-AS1 during neuronal differentiation in our hNPCs culture system, (f) relative expression of significantly downregulated lncRNAs, SPAG5-AS1 and SBTB-AS1 during neuronal differentiation, (g) relative expression of significantly upregulated lncRNAs, RNF-AS1 and CCNT2-AS1 during astrocytic differentiation, (h) relative expression of significantly downregulated lncRNAs, PRKAG-AS1, KRTAP-AS1 and PSMG-AS1 during astrocytic differentiation. *p ≤ 0.05, **p ≤ 0.005, ***p ≤ 0.0005, student t-test.