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Northern blot detection of miRNA candidates m-3350 (a), m-3022 (b), m-4537 (c), m-3558 (d) and m-4731 (e) using radiolabeled probes in HEK293T cells transfected with the respective overexpression constructs. Ctrl indicates transfection of pSG5 vector alone. SYBR Gold (SyGo) or ethidium bromide (EtBr) was used to confirm equal loading. Markers were used to confirm RNA ladders sizes (shown on the left of each gel). Arrows indicate specific signals.
