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. 2019 Jan 28;16(3):340–353. doi: 10.1080/15476286.2019.1572448

Figure 9.

Figure 9.

Localization of PSMB8-AS1 in cells and effects of PSMB8-AS1 on epigenetic markers. (A) The levels of PSMB8-AS1, GAPDH and β-actin mRNAs (cytoplasmic RNA positive controls), and U2snRNA (nuclear RNA positive control) in cytoplasmic and nuclear fractions of A549 cells were determined by real-time PCR. Results are represented for each gene as means ± SE from three independent experiments. (B-D) A549 cells stably expressed dCas9-KRAB were infected with lentiviral gRNAs at a MOI of 200 in the presence of 0.1 µg/ml of puromycin for 48 h and infected with PR/8 at a MOI of 0.01 for 48 h. H3K27m3 and H3K27Ac levels in the nuclei were determined by western blotting and normalized to H3. Representative blots and quantitation were presented in B and C, D, respectively. Results are represented as means ± SE from three independent experiments. No significance was detected by one-way ANOVA.