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. 2019 Jan 15;16(1):118–132. doi: 10.1080/15476286.2018.1564462

Figure 4.

Figure 4.

Effects of YAP1 on cell migration and EMT in DF-1 cells. (a) The overexpression (OE) of YAP1 promoted DF-1 cell migration compared to the cells transfected with empty vector (EV) or the mock-transfected cells. (b) Relative cell migration ability of cells transfected with YAP1, EV- and mock-transfected cells (** represent P < 0.01). (c) The confirmation that YAP1 was successfully overexpressed in DF-1 cells (** represent P < 0.01). Immunoblotting revealed that the overexpression of YAP1 increased the N-cadherin, Fibronectin, Vimentin, ZEB1 and MMP2 protein expression, but decreased the E-cadherin protein expression compared with the mock-transfected cells at 48 h. The levels of YAP1 N-cadherin, Fibronectin, Vimentin, ZEB1, MMP2 and E-cadherin protein are shown as fold change next to the images after normalized with β-actin (** represent P < 0.01). (d) RT-qPCR revealed that the overexpression of YAP1 increased the N-cadherin, Fibronectin, Vimentin, ZEB1 and MMP2 transcription, but decreased the E-cadherin transcription compared with the mock-transfected cells (** represent P < 0.01) at 24 h. The data represent the mean±s.e.m. of three independent experiments.