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. Author manuscript; available in PMC: 2019 Feb 19.
Published in final edited form as: Biochem J. 2018 Apr 16;475(7):1323–1334. doi: 10.1042/BCJ20180044

Figure 2. Depletion of multiple transcription factors enhances CFTR transcript levels in Calu-3 cells.

Figure 2.

siRNA-mediated depletion (KD) of A) BRD8, EHF, KLF5 and B) ING2, NR2F2, enhances CFTR mRNA abundance. RT-qPCR measurements of CFTR normalized to the geometric mean of three house-keeping gene controls, β2 microglobulin (β2M), phosphoglycerate kinase (PGK) and β actin (ACTB) and shown relative to negative control (NC). Bar shows average of 3 experiments with standard error of the mean. Key: *** P<0.001, **** P<0.0001 by an unpaired two-tailed Student’s t test.