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. Author manuscript; available in PMC: 2019 Feb 19.
Published in final edited form as: Biochem J. 2018 Apr 16;475(7):1323–1334. doi: 10.1042/BCJ20180044

Figure 3. Depletion of KLF5, EHF, ING2, NR2F2 and BRD8 enhances CFTR protein abundance in Calu-3 cells.

Figure 3.

siRNA-mediated depletion of BRD8, EHF, KLF5, compared to NCA and ING2, NR2F2, FOXA1/A2 compared to NCD. A) Western blot probed with CFTR antibody 596 (CFF) or β tubulin as a loading control. FOXA1/A2 are known activators of CFTR expression. (NCA = Ambion, NCD =Dharmacon negative controls). B) Quantification of densitometry from ≥3 western blots shows pooled data on the impact of depletion of each factor on CFTR protein abundance. Bar shows average with standard error of the mean. Key: not significant, ns. P>0.05, * P< 0.05, ** P<0.01, **** P<0.0001 by an unpaired two-tailed Student’s t test.