Figure 1. Synapse Formation Produces Persistently Enhanced Bi-directional Mitochondrial Transport in Pre-synaptic Sensory Neurons.
(A) Experimental design.
(B) DIC and fluorescence images are shown. The fluorescence images in boxes show examples of a region where transport was analyzed. MN, motor neuron; NOC, nocodazole; SN, sensory neuron. Scale bar, 10 μm.
(CμF) Bar graphs show the flux and velocity of anterograde (Ant) and retrograde (Ret) mitochondrial transport in SN measured by kymograph analysis. Error bars are SEMs. NS, nonsignificant, *p < 0.05; **p < 0.01; ***p < 0.001. One-way ANOVA and Tukey post hoc test.
(G–I) Bar graphs show the flux and velocity of anterograde and retrograde transport in SN compared to SN co-cultured with L7MN at 6, 12, and 24 h after plating, respectively. Numbers of neurons analyzed are indicated in the bar graphs. Error bars are SEMs. NS, nonsignificant; *p < 0.05. Student’s unpaired t test.