Figure 3.

Fluorescence-activated cell sorting of m13 scFv library expressed on the yeast surface. (a). Cells were first incubated with unbiotinylated SEB, washed in PBST at pH 6.0, and finally incubated with biotinylated SEB at pH 7.4. Bound SEB was detected with SAPE. The cells were also labeled with anti-FLAG antibody and anti-mouse antibody-FITC to impose selection based on the scFv expression. The polygon represents the pool collected during each sorting. (b). The labeling and washing conditions during each round of sorting. The “percentage collected” refers to the number of cells collected divided by the number of cells sorted.