Figure 3.

MiR-21 is a target of GAS5. (a) qRT-PCR analysis of miR-21 expression levels in a panel of RCC cell lines; (b) The effects of GAS5 overexpression/knockdown on miR-21 levels was measured in RCC cells by qRT-PCR; (c) The effects of miR-21 mimics/inhibitor on GAS5 levels was measured in RCC cells by qRT-PCR; (d) Schematic representation of the predicted binding site between miR-21and GAS5 and the mutated site for the reporter assays; (e) Luciferase reporter assay was performed in human embryonic kidney (HEK) 293T cells which was co-transfected with the reporter plasmid (or the corresponding mutant reporter) and the indicated miRNAs; (f) (g) Overexpression of miR-21 abolished GAS5-overexpression induced sorafenib sensitivity. * P < 0.05, ** P < 0.01, ***P < 0.001.