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PDPNlow sorted glioma cells regain high PDPN expression in vivo. (A) PDPN levels indicated by mean fluorescence intensity (MFI) before orthotopic injection (n = 1 per primary tumor) and 2 days after isolation of GBMF2 (n = 2 recipients), GBMF3 (n = 3), and GBMF10 (n = 3) tumor cells. MFI was normalized to PDPNhigh values. (B) Immunohistochemical staining for PDPN, scale bar 1 mm. (C) Survival of mice injected with PDPNlow (n = 6) and PDPNhigh (n = 6) sorted glioma cells.
