Figure 2.

CD47/CD19 co-engagement inhibits BCR-induced cytokines production by human B cells.

Purified human B cells were pretreated with 10 μg/mL hIgG1 isotype control, anti-CD47xCD19 biAb, bivalent anti-CD19 or 10 nM ibrutinib and then stimulated with 5 μg/mL anti-BCR and 1 μg/mL anti-CD40 antibodies. As controls, B cells pretreated with 10 μg/mL hIgG1 isotype control were left unstimulated. After 5 days of incubation at 37°C, supernatants were harvested and IL-6 (a) and TNF (b) levels were measured by a multiplexing assay. Dashed lines represent the lower limit of cytokine detection. Data represent the mean values ± SEM of a minimum of 6 independent donors. Statistical analysis was performed using the one way ANOVA test: **p < 0.01, ***p < 0.001.