Figure 3.
Developability assessment of the antibody solubility library. The variants were analyzed by: (a) Ammonium sulfate precipitation (AMS), (b) hydrophobic interaction chromatography (HIC), (c) cross-interaction chromatography (CIC), (d) stand-up monolayer chromatography* (SMAC*) using an TSK G3000 SWXL column, (e) stand-up monolayer chromatography (SMAC) using an Zenix SEC 300 column, and (f) affinity-capture self-interaction nanoparticle spectroscopy (AC-SINS), and all types of measurements are compared with combined-chain (heavy and light chains) intrinsic CamSol solubility scores (x-axis). Data points are labelled according to the mAb numbering and color coded according to their intrinsic solubility CamSol score as depicted in the color bar. The fits represent linear regressions and the corresponding R2 values are given.