Figure 2.

Reduced Heavy chain LC-MS analysis of glycoforms. Deconvoluted mass spectrometric data of heavy chain of starting mAbs, acceptors and resulting six glycoforms are shown to confirm intended transglycosylation and good agreement with theoretical molecular mass shown in Table 2. Starting mAbs showed relatively heterogeneous mass spectrum, where resulting acceptors and transglycosylated glycoforms showed highly homogeneous profile. Truncation of one sialic acid residue observed for mAb-FA2G2S2 and mAb-A2G2S2 is possibly due to fragmentation induced by ionization. No alternations in light chain were observed by the reduced mass analysis (data not shown).