Figure 7.

Binding affinity of C1q increased by terminal galactosylation and sialylation. (a) Sensorgrams of each glycoform. Glycoforms were first captured on Protein A chip and C1q as analytes were injected by multi-cycle mode at eight concentrations. Sensorgrams were fitted with steady-state equilibrium model. (b) Relative binding activities of C1q. Error bars represent SD of n = 3 and asterisks indicate statistical significance at p < 0.05. (c) JMP analysis: Scaled estimates of effect of glycosylation for binding affinity of C1q. Analyzed for complex biantennary glycoforms and acceptors.