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No effect of glycosylation for binding affinity of FcRn in SPR analysis. (a) Sensorgrams of each glycoform. FcRn was directly immobilized on CM5 chip and glycoforms as analytes were injected by multi-cycle mode at six concentrations. Sensorgrams were fitted with steady-state equilibrium model. (b) Relative binding activities of FcRn. Error bars represent variation range of n = 2.
