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FcRn affinity chromatography showed longer retention by terminal galactosylation and sialylation. FcRn affinity chromatography utilized a human FcRn prepacked column. (a) Chromatograms and (b) Retention time of main peak. Terminal galactosylation and sialylation resulted in longer retention where mAb-HM and partially deglycosylated glycoforms showed the shortened retention. Error bar for mAb-ctrl represents SD of n = 6.
