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. 2018 Dec 10;11(2):350–372. doi: 10.1080/19420862.2018.1551044

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© 2018 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 14. — Long-term forced degradation analyzed by size-exclusion chromatography showed significant increase of aggregation species in acceptors, mAb-HM and terminally galactosylated glycoforms but overall the same level of fragmentation among all glycoforms tested. (a) Chromatographic comparison of glycoforms after incubation for 17 days. (b) % increase of HMW species, with each aggregate-1 and aggregate-2 on top and bottom, respectively. (c) % increase of LMW species, with each fragment-1 and fragment-2 on bottom and top, respectively. n.t: not tested (b-c).