Table 3.
EC50 of each IgG1 glycoforms from ADCC reporter bioassay.
| ng/mL |
Relative effect |
|||||
|---|---|---|---|---|---|---|
| Glycan type | EC50 | 95% CI lower | 95% CI upper | Defucosylation | Galactosylation | Sialylation |
| ctrl | 30.1 | 27.8 | 32.6 | - | - | - |
| FA2G0 | 28.2 | 25.5 | 31.2 | 1.0 | 1.0 | - |
| FA2G2 | 26.5 | 23.2 | 30.4 | 1.0 | 1.1 | 1.0 |
| FA2G2S2 | 28.8 | 26.0 | 31.9 | 1.0 | - | 0.9 |
| A2G0 | 12.6 | 10.1 | 15.5 | 2.2 | 1.0 | - |
| A2G2 | 2.62 | 2.06 | 3.33 | 10.1 | 4.8 | 1.0 |
| A2G2S2 | 4.34 | 3.80 | 4.96 | 6.6 | - | 0.5 |
| HM | 8.96 | 7.48 | 10.7 | - | - | - |
| GlcNAc-Fuc | n.d | n.d | n.d | - | - | - |
| GlcNAc | n.d | n.d | n.d | - | - | - |
n.d: not determined. EC50 of glycoforms calculated from response curves in Figure 6 by four parameter logistic model using Prism 7.