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Fusion with O-ASCs induces EMT in endometrial cancer cells. (a). Immunofluorescent staining for E-cadherin and Vimentin in the hybrid cells and their parental cells. (b). Semi-quantitative expression analysis of E-cadherin and Vimentin was performed by a High-Throughput Imaging Analysis. (c). The expression of EMT-related genes was determined by real-time RT-PCR. The experiments were repeated three times. The statistical results are presented as Means ± SD. *P < 0.05, ***P < 0.001.
