Figure 3.

(A–E) Functional characterization of wt Gαs and variants coexpressed with different GPCRs. 2B2 cells were cotransfected with GPCRs and wt or mutant Gαs, as indicated. After 2 days, cAMP accumulation was determined in the presence of decadic increasing concentration of indicated ligands by α screen technology. Results of four independent experiments performed in triplicates are shown as mean ± SEM. hPTH, human parathyroid hormone; hTSH, human TSH; α-MSH, alpha-melanocyte stimulating hormone.