. 2018 Jul 15;26(11):2984–2991. doi: 10.1016/j.bmc.2018.03.030

Table 1.

AMI derivatives (Fig. 2) and associated Alphascreen IC₅₀ values for displacement of H3K4me3-Bn from His-KDM5A(PHD3). Average ± StdDev (n ≥ 3 independent replicates) shown. H3(1–21)K4me3 IC50: 218 ± 16 nM.

Compounds	A	R₁	R₂	R₃ = R₅	R₄	IC₅₀ (µM)	Biotin-His₆ counterscreen
Amiodarone (AMI)	O	butyl	H	I		99 ± 23	178 ± 58
WAG-003	O	butyl	H	I		1658 ± 490	11280 ± 1258
WAG-005	O	butyl	H	I		562 ± 130	2584 ± 127
1a	O	butyl	H	H		166 ± 20	484 ± 82
2a	O	butyl	H	H		340 ± 44	4297 ± 163
2b	O	methyl	H	H		844 ± 23	6291 ± 1865
2c	S	methyl	H	H		533 ± 109	805 ± 55
2d	NMe	H	H	H		1310 ± 39	970 ± 60
7a	O	butyl		H	H	1146 ± 100	DNC^a
7b	O	methyl		H	H	2305 ± 82	DNC^a
8a	O	butyl	H	OMe		223 ± 60	309 ± 70
8b	O	butyl	H	F		198 ± 50	455 ± 57
9	O	butyl	H	H		NM^b	NM^b
10	O	butyl	H	H		198 ± 41	376 ± 69
12	O	butyl	H	H		314 ± 30	1335 ± 326

DNC: data did not converge to binding model.

NM: not measurable within the solubility range of the compound. Final concentration of protein and peptide were 25 nM.