| Recombinant expression |
|
|
MjpIgR-SC-EX-F |
TACTCAGAATTCGACGCAGACATTATCGTG |
|
MjpIgR-SC-EX-R |
TACTCACTCGAGTGAAAGCCGCACTTTGTA |
|
MjpIgR-IG1-EX-F |
TACTCAGAATTCGACGCAGACATTATCGTG |
|
MjpIgR-IG1-EX-R |
TACTCACTCGAGCTTCACAGTTACGATCAC |
|
MjpIgR-IG2-EX-F |
TACTCAGAATTCGAGACACCAGTTAAGTGT |
|
MjpIgR-IG2-EX-R |
TACTCACTCGAGAGGCACAGTGAGGGTCTT |
|
MjpIgR-IG3-EX-F |
TACTCAGAATTCAGCACTTACTACCAGATT |
|
MjpIgR-IG3-EX-R |
TACTCACTCGAGTGAAAGCCGCACTTTGTA |
|
MjpIgR-ORF-F |
TACTCAGAATTCATGTGCGAGTGTTCCTTTGTG |
|
MjpIgR-ORF-R |
TACTCACTCGAGCTACTCCTTTTTCTCGTCCTCCCG |
|
MjpIgR-ΔIG1-EX-F |
TACTCAGAATTCGACGCAGACATTATCGTG |
|
MjpIgR-ΔIG1-EX-R |
TACTCACTCGAGTCCAGGATGGACATCATC |
|
MjpIgR-In-EX-F |
TACTCAGAATTCCGGTATCGCCAGATGTTC |
|
MjpIgR-In-EX-R |
TACTCACTCGAGTGTCACGGTTCCAACAAT |
|
Mjcalmodulin-EX-F |
TACTCAGGATCCATGGCGGATCAGCTGACCGAA |
|
Mjcalmodulin-EX-R |
TACTCAGTCGACCTTCGAGGTCATCATCGTGAC |
| (q)RT-PCR |
|
|
MjpIgR-RT-F |
AAGTGTTAGCCGACAGGTTG |
|
MjpIgR-RT-R |
CTAAGATTTGTCAGACGCACC |
|
Mjclathrin-RT-F |
ATTTGATAGAGTTTCGTCG |
|
Mjclathrin-RT-R |
GCAGGTCGTAGCAGTGGA |
|
Mjcalmodulin-RT-F |
GGACGCTGACGGTAATG |
|
Mjcalmodulin-RT-R |
GTGGTGGCTGCTTGATA |
| Actin-RT-F |
AGTAGCCGCCCTGGTTGTAGAC |
| Actin-RT-R |
TTCTCCATGTCGTCCCAGT |
| β-integrin-RT-F |
CACATCAGCGTGACCTACA |
| β-integrin-RT-R |
GACGAAAGAACCGAAACC |
| AP2-RT-F |
GCTAAAGAAGAAGAGTGGGAGA |
| AP2-RT-R |
GCAGGTGGAGTTGACAGG |
| VP28-RT-F |
AGCTCCAACACCTCCTCCTTCA |
| VP28-RT-R |
TTACTCGGTCTCAGTGCCAGA |
| RNAi |
|
|
dsMjpIgR-F |
GCGTAATACGACTCACTATAGGCACAAGGAAGGAGTGGAGGTA |
|
dsMjpIgR-R |
GCGTAATACGACTCACTATAGGGGCGGTAACAATAATCAGCA |
|
dsGFP-F |
TAATACGACTCACTATAGGGGGGTGGTCCCAATTCTCGTGGAAC |
|
dsGFP-R |
TAATACGACTCACTATAGGGCTTGTACAGCTCGTCCATGC |
|
dsMjclathrin-F |
GCGTAATACGACTCACTATAGGCCCAACGCTGGTTATGCT |
|
dsMjclathrin-R |
GCGTAATACGACTCACTATAGGTGACCTGACCGCCTCTAC |
|
dsMjcalmodulin-F |
GCGTAATACGACTCACTATAGGTTGTGATACATACACACA |
|
dsMjcalmodulin-R |
GCGTAATACGACTCACTATAGGCGCGGTCACTTCACTTCG |
|
dsβ-integrin-F |
GCGTAATACGACTCACTATAGGCCTGGACAAGAACTCGG |
|
dsβ-integrin-R |
GCGTAATACGACTCACTATAGGCCTGGATAGCACGCACA |
|
dsAP2-F |
GCGTAATACGACTCACTATAGGGAACCTCCTTTCTTCCAACA |
|
dsAP2-R |
GCGTAATACGACTCACTATAGGACACGCAACCCTTATACTCA |
| Overexpression of MjpIgR in nonpermissive cells |
| pIgR-pcDNA3.1(-)-F |
CTACCTCGAGATGTGCGAGTGTTCCTTTGTGC |
| pIgR-pcDNA3.1(-)-R |
GACTGAATTCCTACTCCTTTTTCTCGTCCTCCCG |
| pIgR-ΔIG1-pcDNA3.1(-)-R |
GACTGAATTCTCCAGGATGGACATCATCGTAAAC |
| WSSV VP28-RT-F |
CTCCGCAATGGAAAGTCTGA |
| WSSV VP28-RT-R |
GGGTGAAGGAGGAGGTGTT |
| Genomic DNA F |
CCAACAAGTGTCTCCTCCAAAT |
| Genomic DNA R |
AATCTCCTCAGGGATGTCAAAGT |
