Figure 5.

Soluble and insoluble proteomes. (A) Quantification of keratin 8 in the soluble and insoluble fractions of young and old mice by Western blotting (normalized to total protein reversible stain). (B) Representative Western blot of krt8 and total protein stain. (C) Quantification of the insoluble to soluble intensity ratio of keratin 8 in young and old mice. (D) Heatmap depicting the relative proteomic abundance of proteins in the insoluble fractions versus the soluble fractions for each treatment group, with column and row clustering and dendrograms. Red indicates a greater proportion of insoluble protein and blue indicates a greater proportion of soluble protein. Values are mean ± SEM. Two-tailed t tests: *p < .05. A high quality PDF of Figure 5D is available as a supplementary file.