Fig. 2.

Glycogen Deposits found within Vascular, GI, and GU Smooth Muscle of Gaa^−/− Mouse.

Representative images of fixed and plastic embedded tissue, sectioned to, and stained with Periodic Acid-Schiff reagents to detect glycogen, then counterstained with Toluidine Blue. 2μm sections from the esophagus of six-month-old (A and C) wildtype and (B and D) Gaa^−/− mice are indicative of the smooth muscle found along the vascular system (A and B) as well as within the gastrointestinal tract (C and D). 2μm sections from the bladder of (E) wildtype and (F) Gaa^−/− mice represent the pathology present in the genitourinary. Aggregates of pink puncta indicate large deposits of glycogen within lysosomes. Scale bar = 20 μm.