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. Author manuscript; available in PMC: 2019 Jul 21.
Published in final edited form as: Nat Immunol. 2019 Jan 21;20(3):265–275. doi: 10.1038/s41590-018-0292-y

Figure 7. Metabolic rewiring of TCA cycle supports the oxidative phenotype and anti-tumor activity of CpG-activated macrophages.

Figure 7

BMDMs were pretreated with CpG or vehicle for 96 hours, plated, and then labeled (i) with the addition of 100 μM 13C-palmitate for 4 hours (n=3) (a,b) or (ii) in the presence of 25 mM 13C-glucose or 4 mM 13C-glutamine for 2 hours (n=3) (c-e). (a) Percent isotopologue enrichment of acetyl-CoA with addition of 13C-palmitate. (b) Percent isotopologue enrichment of succinyl-CoA with addition of 13C-palmitate. (c) Relative total pool size of HMG-CoA, succinyl-CoA, and acetyl-CoA after labeling with tracers (13C-glucose or 13C-glutamine). (d) Percent isotopologue enrichment of acetyl-CoA from 13C-glucose and 13C-glutamine. (e) Percent isotopologue enrichment of succinyl-CoA from 13C-glucose and 13C-glutamine. (f) Basal oxygen consumption of BMDMs pretreated as indicated (n=8). (g) In vitro phagocytosis of PDAC.1 CTRL or Cd47–/– cells by BMDMs pretreated as indicated (n=3). Two-tailed Student’s t-test with Hochberg correction: ns (not significant). Plots (a-g) show mean ± s.d. Results are representative of at least two independent experiments (f,g). **, p<0.01; ***, p<0.001; ****, p<0.0001.