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. Author manuscript; available in PMC: 2020 Feb 19.
Published in final edited form as: Environ Sci Technol. 2019 Feb 8;53(4):2114–2123. doi: 10.1021/acs.est.8b05250

Figure 3. Chiral PCB 91, PCB 95, PCB 132 and PCB 136 are oxidized in a congener-specific manner by human CYP2A6 and CYP2B6 to OH-PCBs.

Figure 3.

Representative GC-TOF chromatograms of OH-PCB metabolites (as methylated derivatives) in extracts from incubations of CYP2A6 with (A1) PCB 91; (A2) PCB 95; (A3) PCB 132; and (A4) PCB 136; and in extracts from incubations of CYP2B6 with (B1) PCB 91 and (B2) PCB 132. Incubation conditions were as follow: 50 μM PCB; 60 minutes, 37 ºC; and 10 pmol/mL P450. See the Supporting Information for the corresponding mass spectra. The metabolites were separated on DB5-ms column; see the Experimental Section above for additional details. a No mass spectra were obtained due to low analyte levels. However, the peak was identified by matching the retention time with the authentic standard. b Accurate mass not determined due to background carbon interference. RS, peak corresponding to the recovery standard.