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. Author manuscript; available in PMC: 2020 Feb 19.
Published in final edited form as: Environ Sci Technol. 2019 Feb 8;53(4):2114–2123. doi: 10.1021/acs.est.8b05250

Figure 5. OH-PCB metabolites are formed atropselectively from (A) PCB 91, (B) PCB 95, (C) PCB 132 and (D) PCB 136 in incubations with recombinant human CYP2A6 (dark red), CYP2B6 (orange) and CYP2E1 (light green) enzymes.

Figure 5.

No metabolites were detected in experiments with CYP1A2 and CYP3A4. Open circles (○), diamonds (◇) and squares (□) indicate 1,2-shift, meta- and para-substituted metabolites, respectively. Data are expressed as mean ± SD, n = 3; error bars are typically hidden behind the symbols. Metabolism studies were performed using the following incubation conditions: 50 μM PCB; 60 min incubation at 37 ºC; 10 pmol/mL P450 content; and ~1 mM NADPH regenerating system. Metabolites were separated on BDM (5–91, 4–91, 3–103 and 4’−95); GTA (3–100, 3’−140) or CD columns (5’−132, 3–150, 5– 136 and 4–136). EF value of 5–95 and 4–95 could not be calculated due to co-elution of E1-5–95 and E1-4–95.