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(A) Ribonucleolytic activity of RNase 1 and its glycoforms. Values of kcat/KM were determined for the cleavage of 6-FAM–dArU(dA)2–6-TAMRA at pH 7.5 and 25 °C. Each data point represents an individual measurement. (B) Thermostability of RNase 1 and its glycoforms. Values of Tm were determined were determined by using DSF in PBS containing SYPRO Orange (50×). Each data point represents an individual measurement.
