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SDS–PAGE gels showing the effect of trypsin on RNase 1 glycoforms. An RNase 1 glycoform (1 mg/mL) was incubated with trypsin (0.1, 1, or 10 µg/mL) at 37 °C for 1 h. The trypsin was inactivated with PMSF, and the products were subjected to electrophoresis in a 15% w/v gel and visualized by staining with Coomassie blue.
