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. Author manuscript; available in PMC: 2020 Feb 19.
Published in final edited form as: Biochemistry. 2019 Jan 31;58(7):951–964. doi: 10.1021/acs.biochem.8b01253

Figure 11. Oligomeric state and purity of MyOobT.

Figure 11.

A. SDS-PAGE behavior of MjCobT compared to BioRad Precision Plus Protein Standards. Based on the data shown in panel B, MjCobT was estimated to be >99% homogeneous. B. Sedimentation velocity c(s) distribution of MjCobT at pH7.5 showing three species at 0.75 S, 4.42 S, and 6.51 S C. Cartoon model of the MjCobT dimer (cyan) identified in the crystal structure (PDB ID 3L0Z) superimposed onto the homologous CobT from Pyrococcus horikoshii dimer in grey (PDB ID 3U4G).