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. Author manuscript; available in PMC: 2020 Feb 19.
Published in final edited form as: Biochemistry. 2019 Jan 31;58(7):951–964. doi: 10.1021/acs.biochem.8b01253

Figure 7. Effect of pH on MjCobT activity.

Figure 7.

Conditions for the PRTase reaction are described under Materials and Methods. Product formation is displayed as a function of pH. Error bars represent the standard error of the mean. Asterisks represent significance determined by unpaired T test compared to pH 7. The following buffers were tested in order of appearance: sodium malate (pH 5), sodium succinate (pH 5.5), MES (pH 6), 2-[Bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol (Bis-Tris, pH 6.5), imidazole (pH 7), phosphate (pH 8), 2-amino-2-(hydroxymethyl)-1,3-propanediol chloride (Tris-HCl pH 7, 8, 9), N-cyclohexyl-2-aminoethanesulfonic acid (CHES pH 9, 9.5), and glycine (pH 10, 10.5).