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. Author manuscript; available in PMC: 2020 Feb 19.
Published in final edited form as: Biochemistry. 2019 Jan 31;58(7):951–964. doi: 10.1021/acs.biochem.8b01253

Figure 9. MjCobT activates 5,6- dimethylbenzimidazole to α-ribazole- phosphate (α-RP).

Figure 9.

Conditions for the PRTase reaction are described under Materials and Methods. In panel A, product formation is shown as a function of time across varying NaMN concentrations. Product formation was quantified as a function of time. Error bars represent standard error of the mean. Asterisks shown significance determined by unpaired T test comparing time 0 to time 15. Panel B shows average specific activities with standard deviations for each concentration of NaMN tested.