Table 1.

Doubling times (h) of a S. enterica cobT cobB strain harboring plasmids encoding MjCobT proteins

Variant (plasmid)	Cbi (15 nM)	Cbi + DMB	Cbi + Ade	Cbl (100 nM)
Vector (pBAD24)	NG	NG	NG	1.2 ± 0.01[3 h lag]
MjCobTWT (pMjCobT1)	1.6 ± 0.01 [3 h lag]	1.4 ± 0.01, [3 h lag] (10 nM)	1.5 ± 0.01, [3 h lag] (10 nM)	1.2 ± 0.01, [3 h lag]
MjCobTE150A (pMjCobT5)	NG	2.0 ± 0.02, [3 h lag] (50 μM) 3.4 ± 0.12, [20 h lag] (1 μM)	NG (1mM)	1.5 ± 0.01, [4 h lag]
MjCobTE315A (pMjCobT3)	NG	1.6 ± 0.01, [3 h lag] (5 μM) 2.0 ± 0.19[19 h lag] (50 nM)	2.2 ± 0.03, [6 h lag] (0.5 mM)	1.8 ± 0.03, [4 h lag]
MjCobTE150A E315A (pMjCobT4)	NG	NG	NG	1.9 ± 0.17, [4 h lag]

Cells were grown in NCE minimal medium supplemented with ribose (20mM) as the sole carbon and energy source. Doubling times are shown in hours with the standard error of the mean. Lag times are shown in brackets. Concentrations of added ring precursor or base are shown in parenthesis. NG, no growth; Cbi, dicyanocobinamide [(CN)₂Cbi]; DMB, 5,6-dimethylbenzimidazole; Cbl, cyanocobalamin (CNCbl, vitamin B₁₂); Ade, adenine