Figure 10.

The preventive effects of peptides 1, 5, and 6 against vaso-obliteration in an OIR model. Five-days old Sprague Dawley pups and their mothers were kept in 80% oxygen until the 10 day of life, receiving twice-daily intraperitoneal injections of peptides 1, 5, and 6 (2 mg/kg/day), Kineret (15 mg/kg/day) or PBS vehicle (each injection was titrated to a volume of 20 μL). (A) Representative retinal flatmounts stained with FITC-conjugated Bandeiraea simplicifolia lectin, taken at 10X magnification and stitched with MosaiX in Axiovision 4.8. Yellow lines indicate the central area of vaso-obliteration extending from the optic nerve (center of each retina), scale bar 2 mm. (B) Quantification of area of vaso-obliteration performed using ImageJ, expressed as a percentage of the total retinal area: n = 5–7 of peptides 5 and 6 and Kineret, n = 10–12 for vehicle and peptide 1; ^**p < 0.01, ^****p < 0.0001 relative to the vehicle group. Treatment groups that are not labeled with asterisks are statistically non-significant compared to the vehicle group.