Figure 11.

The effects of peptides 1, 5, and 6 on retinal microglial activation and density. Retinas were obtained for immunohistochemistry from rat pups treated with the OIR protocol, and incubated with rabbit anti-iba-1 antibody, followed by donkey anti-rabbit antibody conjugated to Alexa 594. (A) Representative confocal images of retinal microglia at 30X magnification: scale bar 100 μm. (B) Epifluorescence microscopy images at 20 × magnification of retinal microglial density quantified using ImageJ: 4 images per retina were taken at a distance halfway between the optic nerve and the peripheral edge of the retina; n = 5–7 for peptide 1, 5, and 6, and Kineret; n = 8–10 for normoxia and vehicle; ^*p < 0.05, ^****p < 0.0001 relative to the vehicle group. Treatment groups that are not labeled with asterisks are statistically non-significant compared to the vehicle group.