Figure 3.

Tissue infiltration of T cells and hemophagocytic macrophages in huSGM3 mice. Liver and lung tissue samples were prepared from huNSG and huSGM3 mice 18–22 weeks after transplantation, and examined histologically (n = 4 per group). (A) Representative H&E staining of liver and lung tissue sections from huNSG and huSGM3 mice (without human thymus). Original magnification: x50. Arrow signs in huSGM3 mice organs show severe cellular infiltrations. (B) Representative staining images infiltration of hemosiderin-containing hemophagocytic histiocytes (macrophages) in liver and lungs from huSGM3 mice (without human thymus). Hemosiderin-containing hemophagocytic histiocytes, indicated by arrow signs, are stained with brown by H&E and blue by Prussian blue. *denotes a representative giant cell (i.e., fused macrophages) containing ingested erythrocytes. Original magnification: x200. (C) Representative immunohistochemical staining of huSGM3 mouse (without human thymus) liver sections (two consecutive sections) with anti-human CD3 and anti-human CD68 antibodies. *denotes a CD68⁺ giant cell.