Figure 5.

Cytopenia and bone marrow hypoplasia in huSGM3 mice. HuSGM3 mice with (closed square) or without (open square) human thymus were sacrificed when moribund between 18 and 22 weeks. HuNSG mice with (closed circle) or without (open circle) human thymus were sacrificed at 21–22 weeks after transplantation as controls. Since there was no apparent difference between the “thymus+” and “thymus-” groups, the data were pooled (but samples from different groups are distinguishable by their respective symbols). (A) White blood cell count (WBC), hemoglobin concentration, platelet count, and reticulocyte count measured by hematometry. Unpaired t-test; **P < 0.01; ***P < 0.001. Error bars indicate SEMs. (B) Histology of BM analyzed by H&E staining and cytology of BM analyzed by Wright-Giemsa staining. (C) Absolute cell numbers of human CD45⁺ cells, CD33⁺ myeloid cells and CD3⁺ T cells in BM, which were calculated by multiplying the total cell count after mononuclear cell-purification using density gradient centrifugation with the percentage of each cell population determined by flow cytometry. One-way ANOVA with Bonferroni's post-hoc multiple comparison test; each symbol represents an individual mouse. **P < 0.01.