Figure 3.

Paramyxovirus infections induce SERCA expression. (A) PPRV infection induces SERCA2 expression: Vero cells were infected with PPRV at MOI 5, and the cell lysates were prepared at indicated time points. The levels of SERCA2 (upper panel) or house-keeping control protein (GAPDH) (lower panel) were examined by Western blot analysis. (B) Thapsigargin inhibits NDV-induced SERCA2 expression in Vero cells: Vero cells were infected with NDV at MOI of 5 for 1 h followed by washing with PBS and addition of fresh medium containing Thapsigargin or vehicle control (0.05% DMSO). Cell lysates were prepared at 16 hpi to probe SERCA2 and GAPDH by Western blot analysis. Relative fold-change in the levels of viral/cellular proteins was determined by ImageJ (NIH).