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. 2019 Feb 13;10:209. doi: 10.3389/fmicb.2019.00209

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Copyright © 2019 Kumar, Khandelwal, Kumar, Chander, Rawat, Chaubey, Sharma, Singh, Riyesh, Tripathi and Barua

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Time-of-addition assay. Confluent monolayers of Vero cells were infected, in triplicate, with PPRV or NDV at MOI of 5, washed six times with PBS and fresh medium with either 0.5 μM Thapsigargin or 0.05% DMSO were added at indicated times. Supernatants were collected at 12 (NDV) or 48 hpi (PPRV) and quantified by plaque assay. Time-of-addition assay for NDV (A) and PPRV (B) is shown.