Figure 7.

Thapsigargin treatment induces enhanced formation of cytoplasmic foci. Vero cells at ~20% confluence were infected with NDV or PPRV at MOI of 10. At 3 (NDV) or 10 hpi (PPRV), cells were incubated with either 0.5 μM Thapsigargin or 0.05% DMSO. At 10 (NDV) or 36 hpi (PPRV), cells were fixed and subjected for immunofluorescence assay for localization of the viral proteins in the virus-infected cells. Virus was stained with FITC (green), whereas SERCA was stained with rhodamine conjugate. DAPI was used as nuclear stain. Subcellular localization of NDV (A) and PPRV (B) is shown. Virus-infected cells (FITC) were usually of two types – with or without punctuate structures. To quantitate the subcellular localization, 100 cells (under several different fields) were randomly counted. The percentage of the cells with or without punctuate structures in Thapsigargin-treated and control-treated is shown for NDV (C) and PPRV (D).