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. 2019 Feb 13;10:209. doi: 10.3389/fmicb.2019.00209

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Copyright © 2019 Kumar, Khandelwal, Kumar, Chander, Rawat, Chaubey, Sharma, Singh, Riyesh, Tripathi and Barua

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nucleotide sequencing of passaged virus. RNA was extracted from P70-Control and P70-Thapsigargin viruses as well as plaque purified viruses (two plaques each of P70-Control and P70-Thapsigargin) followed by amplification and sequence analysis of F gene. Nucleotide sequences were translated into amino acid sequences and compared with P0 as well as WT sequences retrieved from GenBank (n = 18). Amino acid mutations associated with Thapsigargin resistance as well as those acquired simply due to sequential cell culture passage (present in both P70-Control and P70-Thapsigargin) are shown.