Table 2.
Tested isolates and results for the real-time PCR.
| Isolates | Source | Targeted gene loci | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| rfbE | prfA | hilA | toxR | scpA | foxA | ddl | ipaH | ureR | toxR | ebpS | mapA | ||
| ESCHERICHIA SPP. (N = 18) | |||||||||||||
| Escherichia coli O157:H7 | ATCCa35150 | + | – | - | – | – | – | – | – | – | – | – | – |
| Escherichia coli O157:H7 | NCTCb12900 | + | – | – | – | – | – | – | – | – | – | – | – |
| Escherichia coli O157:H7 | CICCc21531 | + | – | – | – | – | – | – | – | – | – | – | – |
| Escherichia coli O157:H7 | Stored in our laboratory | + | – | – | – | – | – | – | – | – | – | – | – |
| Escherichia coli O26 | Stored in our laboratory | – | – | – | – | – | – | – | – | – | – | – | – |
| Escherichia coli O138 | Stored in our laboratory | – | – | – | – | – | – | – | – | – | – | – | – |
| Escherichia coli O139 | Stored in our laboratory | – | – | – | – | – | – | – | – | – | – | – | – |
| Escherichia coli | ATCC13706 | – | – | – | – | – | – | – | – | – | – | – | – |
| Escherichia coli | ATCC25922 | – | – | – | – | – | – | – | – | – | – | – | – |
| Escherichia coli | CMCCd44113 | – | – | – | – | – | – | – | – | – | – | – | – |
| Escherichia coli | CMCC44505 | – | – | – | – | – | – | – | – | – | – | – | – |
| Escherichia coli | CMCC44216 | – | – | – | – | – | – | – | – | – | – | – | – |
| Escherichia coli | CMCC44338 | – | – | – | – | – | – | – | – | – | – | – | – |
| Escherichia coli | CMCC44336 | – | – | – | – | – | – | – | – | – | – | – | – |
| Escherichia coli | CMCC44110 | – | – | – | – | – | – | – | – | – | – | – | – |
| Escherichia coli | CMCC44824 | – | – | – | – | – | – | – | – | – | – | – | – |
| Escherichia coli | CMCC44825 | – | – | – | – | – | – | – | – | – | – | – | – |
| Escherichia coli | CGMCCe1.8732 | – | – | – | – | – | – | – | – | – | – | – | – |
| LISTERIA SPP. (N = 7) | |||||||||||||
| Listeria monocytogenes | ATCC19118 | – | + | – | – | – | – | – | – | – | – | – | – |
| Listeria monocytogenes | CMCC54001 | – | + | – | – | – | – | – | – | – | – | – | – |
| Listeria monocytogenes | Stored in our laboratory | – | + | – | – | – | – | – | – | – | – | – | – |
| Listeria monocytogenes | ATCC19115 | – | + | – | – | – | – | – | – | – | – | – | – |
| Listeria ivanovii | ATCC19119 | – | + | – | – | – | – | – | – | – | – | – | – |
| Listeria ivanovii | C4(20031122) | – | + | – | – | – | – | – | – | – | – | – | – |
| Listeria welshimeri | GDMCCf1.232 | – | – | – | – | – | – | – | – | – | – | – | – |
| Listeria innocua | ATCC33090 | – | – | – | – | – | – | – | – | – | – | – | – |
| SALMONELLA SPP. (N = 12) | |||||||||||||
| Salmonella enterica subsp.enterica | Stored in our laboratory | – | – | + | – | – | – | – | – | – | – | – | – |
| Salmonella enterica subsp.enterica | ATCC14028 | – | – | + | – | – | – | – | – | – | – | – | – |
| Salmonella enterica serovar Choleraesuis | CMCC50306 | – | – | + | – | – | – | – | – | – | – | – | – |
| Salmonella enterica serovar Typhimurium | CMCC50115 | – | – | + | – | – | – | – | – | – | – | – | – |
| Salmonella enterica serovar Senftenberg | CMCC50315 | – | – | + | – | – | – | – | – | – | – | – | – |
| Salmonella enterica serovar Aberdeen | CMCC50312 | – | – | + | – | – | – | – | – | – | – | – | – |
| Salmonella enteria serovar Aberdeen | CMCC50313 | – | – | + | – | – | – | – | – | – | – | – | – |
| Salmonella enterica serovar Paratyphi | CMCC50774 | – | – | + | – | – | – | – | – | – | – | – | – |
| Salmonella enterica serovar Rubislaw | CMCC50798 | – | – | + | – | – | – | – | – | – | – | – | – |
| Salmonella entericca serovar Champaign | CMCC50067 | – | – | + | – | – | – | – | – | – | – | – | – |
| Salmonella enterica serovar Paratyphi A | CMCC50093 | – | – | + | – | – | – | – | – | – | – | – | – |
| Salmonella enterica serovar Paratyphi B | CMCC50094 | – | – | + | – | – | – | – | – | – | – | – | – |
| VIBRIO SPP. (N = 12) | |||||||||||||
| Vibrio fluvialis | ATCC33812 | – | – | – | + | – | – | – | – | – | – | – | – |
| Vibrio fluvialis | ATCC33809 | – | – | – | + | – | – | – | – | – | – | – | – |
| Vibrio fluvialis | ATCC33810 | – | – | – | + | – | – | – | – | – | – | – | – |
| Vibrio fluvialis | CGMCC1.1610 | – | – | – | + | – | – | – | – | – | – | – | – |
| Vibrio parahaemolyticus | ATCC17802 | – | – | – | – | – | – | – | – | – | + | – | – |
| Vibrio parahaemolyticus | CMCC20502 | – | – | – | – | – | – | – | – | – | + | – | – |
| Vibrio parahaemolyticus | CMCC20516 | – | – | – | – | – | – | – | – | – | + | – | – |
| Vibrio alginolyticus | ATCC17749 | – | – | – | – | – | – | – | – | – | – | – | – |
| Vibrio vulnificus | ATCC27562 | – | – | – | – | – | – | – | – | – | – | – | – |
| Vibrio vulnificus | CGMCC1.8674 | – | – | – | – | – | – | – | – | – | – | – | – |
| Vibrio cholerae | GIM1.449 | – | – | – | – | – | – | – | – | – | – | – | – |
| vibrio proteolyticus | ATCC15338 | – | – | – | – | – | – | – | – | – | – | – | – |
| STREPTOCOCCUS SPP. (N = 5) | |||||||||||||
| Streptococcus pyogenes | ATCC19615 | – | – | – | – | + | – | – | – | – | – | – | – |
| β-Hemolytic streptococcus | CMCC32210 | – | – | – | – | + | – | – | – | – | – | – | – |
| β-Hemolytic streptococcus | CMCC32204 | – | – | – | – | + | – | – | – | – | – | – | – |
| Streptococcus pneumoniae | ATCC49619 | – | – | – | – | – | – | – | – | – | – | – | – |
| Streptococcus thermophilus | CGMCC1.6472 | – | – | – | – | – | – | – | – | – | – | – | – |
| YERSINIA SPP. (N = 9) | |||||||||||||
| Yersinia enterocolitica | CMCC52225 | – | – | – | – | – | + | – | – | – | – | – | – |
| Yersinia enterocolitica | CMCC52219 | – | – | – | – | – | + | – | – | – | – | – | – |
| Yersinia enterocolitica | CMCC52301 | – | – | – | – | – | + | – | – | – | – | – | – |
| Yersinia enterocolitica | CMCC52302 | – | – | – | – | – | + | – | – | – | – | – | – |
| Yersinia enterocolitica | CMCC52203 | – | – | – | – | – | + | – | – | – | – | – | – |
| Yersinia enterocolitica | CMCC52206 | – | – | – | – | – | + | – | – | – | – | – | – |
| Yersinia enterocolitica | ATCC23715 | – | – | – | – | – | + | – | – | – | – | – | – |
| Yersinia pseudotuberculosis | CMCC53504 | – | – | – | – | – | – | – | – | – | – | – | – |
| Yersinia intermedia | CGMCC1.6197 | – | – | – | – | – | – | – | – | – | – | – | – |
| ENTEROCOCCUS SPP. (N = 5) | |||||||||||||
| Enterococcus faecalis | ATCC19433 | – | – | – | – | – | – | + | – | – | – | – | – |
| Enterococcus faecalis | ATCC29212 | – | – | – | – | – | – | + | – | – | – | – | – |
| Enterococcus faecalis | CMCC32001 | – | – | – | – | – | – | + | – | – | – | – | – |
| Enterococcus faecalis | CICC20419 | – | – | – | – | – | – | + | – | – | – | – | – |
| Enterococcus faecalis | ATCC700802 | – | – | – | – | – | – | + | – | – | – | – | – |
| SHIGELLA SPP. (N = 15) | |||||||||||||
| Shigella spp. | ATCC12038 | – | – | – | – | – | – | – | + | – | – | – | – |
| Shigella flexneri | ATCC12022 | – | – | – | – | – | – | – | + | – | – | – | – |
| Shigella flexneri | CMCC51066 | – | – | – | – | – | – | – | + | – | – | – | – |
| Shigella flexneri | CMCC51571 | – | – | – | – | – | – | – | + | – | – | – | – |
| Shigella flexneri | CMCC51508 | – | – | – | – | – | – | – | + | – | – | – | – |
| Shigella flexneri | ATCC12022 | – | – | – | – | – | – | – | + | – | – | – | – |
| Shigella flexneri | CMCC51067 | – | – | – | – | – | – | – | + | – | – | – | – |
| Shigella dysenteriae | CMCC51135 | – | – | – | – | – | – | – | + | – | – | – | – |
| Shigella dysenteriae | CMCC51336 | – | – | – | – | – | – | – | + | – | – | – | – |
| Shigella dysenteriae | CMCC51252 | – | – | – | – | – | – | – | + | – | – | – | – |
| Shigella sonnei | CMCC51424 | – | – | – | – | – | – | – | + | – | – | – | – |
| Shigella sonnei | CMCC51081 | – | – | – | – | – | – | – | + | – | – | – | – |
| Shigella sonnei | ATCC25931 | – | – | – | – | – | – | – | + | – | – | – | – |
| Shigella boydii | CMCC51515 | – | – | – | – | – | – | – | + | – | – | – | – |
| Shigella boydii | CMCC51510 | – | – | – | – | – | – | – | + | – | – | – | – |
| PROTEUS SPP. (N = 8) | |||||||||||||
| Proteus mirabilis | ATCC35659 | – | – | – | – | – | – | – | – | + | – | – | – |
| Proteus mirabilis | CMCC49005 | – | – | – | – | – | – | – | – | + | – | – | – |
| Proteus vulgaris | CMCC49027 | – | – | – | – | – | – | – | – | – | – | – | – |
| Proteus vulgaris | ACCCg11002 | – | – | – | – | – | – | – | – | – | – | – | – |
| Proteus vulgaris | CMCC49001 | – | – | – | – | – | – | – | – | – | – | – | – |
| Proteus vulgaris | CMCC49107 | – | – | – | – | – | – | – | – | – | – | – | – |
| Proteus vulgaris | CMCC49101 | – | – | – | – | – | – | – | – | – | – | – | – |
| Proteus penneri | ATCC33519 | – | – | – | – | – | – | – | – | – | – | – | – |
| STAPHYLOCOCCUS SPP. (N = 7) | |||||||||||||
| Staphylococcus aureus | Stored in our laboratory | – | – | – | – | – | – | – | – | – | – | + | – |
| Staphylococcus aureus | ATCC43300 | – | – | – | – | – | – | – | – | – | – | + | – |
| Staphylococcus aureus | ATCC29213 | – | – | – | – | – | – | – | – | – | – | + | – |
| Staphylococcus aureus | ATCC27217 | – | – | – | – | – | – | – | – | – | – | + | – |
| Staphylococcus aureus | ATCC6538 | – | – | – | – | – | – | – | – | – | – | + | – |
| Staphylococcus epidermidis | ATCC14990 | – | – | – | – | – | – | – | – | – | – | – | – |
| Staphylococcus epidermidis | ATCC49134 | – | – | – | – | – | – | – | – | – | – | – | – |
| CAMPYLOBACTER SPP. (N = 2) | |||||||||||||
| Campylobacter jejuni | ATCC33252 | – | – | – | – | – | – | – | – | – | – | – | + |
| Campylobacter jejuni | ATCCBAA−1153 | – | – | – | – | – | – | – | – | – | – | – | + |
| OTHER (N = 5) | |||||||||||||
| Klebsiella peneumoniae | CMCC46117 | – | – | – | – | – | – | – | – | – | – | – | – |
| Enterobacter Sakazakii | CMCC45401 | – | – | – | – | – | – | – | – | – | – | – | – |
| Bacillus cereus | CMCC63302 | – | – | – | – | – | – | – | – | – | – | – | – |
| Pseudomonas aeruginosa | ATCC9027 | – | – | – | – | – | – | – | – | – | – | – | – |
| Clostridium perfringens | ATCC13124 | – | – | – | – | – | – | – | – | – | – | – | – |
a
ATCC, American Type Culture Collection, USA;
b
NCTC, National Collection of Type Cultures, U.K.;
c
CICC, China Center of Industrial Culture Collection;
d
CMCC, China Medical Culture Collection;
e
CGMCC, China General Microbiological Culture Collection Center;
f
GDMCC, Guangdong Microbial Culture Center;
g
ACCC, Agricultural Culture Collection of China.