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. 2019 Feb 19;9:2288. doi: 10.1038/s41598-018-38296-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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α-syn aggregation does not induce cell death. (A) Western blot analysis of live cell lysates collected at different time points following osmotic shock. (B) Western blot analysis of dead cell lysates collected at different time points following osmotic shock. (C) Bar graph demonstrating the percentage of α-syn aggregation/actin, with data presented from the live cells and dead cells analysis (panel A & B, respectively). (D) An example of the FACS plots derived from cell death experiments.