Fig. 3.

The effects of kindlin-2 depletion on PYCR, P5CS, and PRODH levels. a A549 cells were infected with control (Sh-NC) or kindlin-2 shRNA (Sh-K2) lentivirus for 5 days and then analyzed by western blot with antibodies recognizing kindlin-2, PYCR1, PYCR2, PYCRL, P5CS, PRODH, and GAPDH (as a loading control). Three independent experiments were shown. b Protein levels of kindlin-2, PYCR1, PYCR2, PYCRL, P5CS, and PRODH relative to that of GAPDH in Sh-NC and Sh-K2 cells were quantified by densitometric analysis of western blottings and compared with those in the A549 cells (normalized to 1, n = 3). Data represent mean ± SEM from three independent experiments using one-way ANOVA with Tukey–Kramer post-hoc analyses, ***p < 0.001, NS, no significance